Biotransformasi Tartrazin oleh Bakteri Usus Manusia

Melvariani Syari Batubara(1*), Nurmaini Ginting(2),

(1) Universitas Muhammadiyah Tapanuli Selatan
(2) Universitas Muhammadiyah Tapanuli Selatan
(*) Corresponding Author


The aimed of this research was to investigated human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri), to investigated dose of tartrazine, and to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The method of this research was experiment with significant correlation to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). This study is primarily focussed on made bacteria medium, made standart inoculums of bacteria, and evaluation for activity of human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The results obtained were attributed to the human intestinal bacteria was used in this research was biotransformated tartrazine. From the results that had been increase dose of tartrazine correlation with increase biotransformation by human intestinal bacteria. Highest dose of tartrazine was 15 mg, and highest biotransformation by Vibrio fischeri, which rate of zone decolourisation diameter was 1,22 cm, and rate of zone decolourisation was 1,44 cm2. Statistically showed significantly in correlation dose of tartrazine with rate of zone decolourisation (0,001 < P < 0,05), but statistically showed non significantly in correlation bacteria with rate of zone decolourisation (0,05 < P < 0,268).


Tartrazine, Biotransformation, Human Intestinal Bacteria

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Batubara, M.S., Siregar, Y., Rusmarilin, H., Soviani, S., Febriani, H.,. (2017). Hubungan Kadar Tartrazin dan Senag (ZN) Dalam Darah Pada Anak Penderita Defisit Perhatian dan Gangguan Hiperaktivitas (ADHD), BioLink, Vol. 4 (2): Hal. 1-10.

Blakeman, D. (2009). Tartrazine. Blends Colour And Flavours For Food & Drink.

Chairul, M. Harapini, dan Y. Daryati. (1996). Pengaruh Ekstrak Kencur (Kaempferia galanga L. ) Terhadap Kehamilan Mencit Putih (Mus musculus). Seminar Nasional Indonesia IV. Jakarta ; Lab. Treub Puslitbang Biologi LIPI, Bogor dan Fakultas Farmasi Universitas 17 Agustus 1945

Chung, King-Thorn, S.E. Stevens and C. E. Cerniglia. (2006). The Reduction of Azo Dyes by the Intestinal Microflora. Critical Reviews in Microbiology. 18 (3) : p. 175-190.

Das, A and A. Mukherjee. (2006). Genotoxicity Testing of the Food Colours Amaranth and Tartrazine. Int. J. Hum. Genet. 4 (4) : p. 277-280.

Dwidjoseputro, D. (2010). Dasar-Dasar Mikrobiologi. Penerbit Djambatan : Malang.

Edicol Colour. (2009). Tartrazine. European Food Safety Authority (EFSA) Panel on Food Additives and Nutrient Sources added to Food (ANS). (2009). Scientific Opinion on the re-evaluation Tartrazine (E 102). EFSA Journal. 7 (11) : p. 1331. Flavour Master Limited. (2009). Tartrazine.

Gottlieb, A., C. Shaw, A. Smith, A. Wheatly and S. Forsythe. (2006). The Toxicity of Textile Reactive Azo Dyes after Hydrolysis and Decolourisation. Journal of Biotechnology. 101 (1) : p. 49-56.

Jervis, L. and C. Northcott. (2006). Metabolism of Tartrazine by Haloperoxidase.

McCann, J., Ames, B. N., Yamasaki, E. (2007). Methods for Detecting Carcinogens and Mutagens with Salmonellal Mammalian Microsome Mutagenicity Test. Mutat. Res. 31 : p. 347-364.

Nakayama, T., Kimura, T., Kodama, M., and Nagata, C. (2007). Generation of Hydrogen Peroxide and Superoxide Anions from Active Metabolites of Naphthalamines and Aminoazo Dyes : Its Possible Role in Carcinogenesis. Carcinogenesis. 4 : p. 765-769.

Oranusi, N. A. and Njoku, H. O. (2006). Biotransformation of Food Dyes by Human Intestinal Bacteria (Streptococcus faecalis, Eschericia coli). J. Appl. Sci. Environ. Mgt. 10 (2) : p. 159-163.

Pelczar, M. J. dan E. C. S. Chan. 2010. Dasar-Dasar Mikrobiologi. Penerbit Universitas Indonesia : Jakarta.

Sasaki, Y. F., Kawaguchi, S., Kayama, A., Ohshita, M. Kabasawa, K. and Iwama, K. (2007). The Comet Assay with 8 Mouse Organs : Results with 39 Currently Used Food Additives. Mutat. Res. 519 : p. 103-109.

Staf Pengajar Fakultas Kedokteran Universitas Indonesia. (2010). Buku Ajar Mikrobiologi Kedokteran. Edisi Revisi. Penerbit Binarupa Aksara : Jakarta.

Sweeney, E. A., J. K. Chipman and S. J. Forsythe. (2006). Evidence for Direct-acting Oxidative Genotoxicity by Reduction Product of Azo Dyes. Environ. Health Perspect. 102 (6) : p. 119-122.

Tanaka, T. (2007). Reproductive and Neurobehavioral Toxicity Study of Tartrazine Administered to Mice in the Diet. Food and Chemical Toxicology Journal. 1 (1) : p. 1-9.

The South African Association For Food Science And Technology. (2009). Tartrazine.


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