DETEKSI BAKTERI GENUS VIBRIO SEBAGAI CAUSATIVE AGENT PADA IKAN LELE SANGKURIANG (CLARIAS GARIEPINUS VAR. SANGKURIANG) DI KOTA TASIKMALAYA


Vita Meylani(1*), Rinaldi Rizal Putra(2),


(1) Universitas Siliwangi
(2) Universitas Siliwangi
(*) Corresponding Author

Abstract


Sangkuriang catfish (Clarias gariepinus var Sangkuriang) is the main commodity that widely cultivated in Tasikmalaya City. However, farmers have difficulty because of the disease which causes death in fish. High mortality is suspected as a vibriosis disease by genus Vibrio because redness wounds on dead fish. The purpose of this study is to know the genus Vibrio which causes of disease in Sangkuriang catfish. 10 samples of fish were taken from Sangkuriang catfish pond culture in Kelurahan Kersanagara Tasikmalaya City which were potentially suspected of vibriosis disease. Isolation of bacteria were done on TCBS medium. Bacterial isolates were collected from fish lesion on the body surface, liver, and kidneys of catfish. Isolation were able to gained 21 isolates and then 5 isolates (VK1, VK5, VK7, VK17, and VK21) were selected based on colony morphology and Postulates Koch’s were tested. The results showed that the clinical symptoms of catfish infected by vibriosis were redness lesions/ulcers on the body surface, hemorrhagic, fluid inside stomach, and fin eroded with redness wound. Bacterial identification through biochemical test revealed the causative agent of catfish disease at brackish pond area were bacteria of the genus Vibrio (VK 1, VK 5, and VK 7), Vibrio vulnificus (VK 17 and VK 21).

Keywords


causative agent, Clarias gariepinus var. Sangkuriang, genus Vibrio

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Sarjito, Ningrum, N.E.W., Radjasa, O.K., dan Prayitno, S.B., 2012. Appication of Repetitive Sequence Base PCR on The Richness of Vibrio on The Tiger Shrimps (Penaeus monodon F.) . Jurnal of Coastal Development, 15 (3) : 304 – 310

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Sarjito, Prayitno, S.B., Radjasa O.K dan Hutabarat, S. 2007. Causative Agent Vibriosis pada Kerapu Bebek (Cromileptes Altivelis) dari Karimunjawa 1. Pathogenisitasnya terhadap Ikan Kerapu Macan (Epinephelus fuscoguttatus). Jurnal Ilmu Kelautan, 12(3) : 173 – 180

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Sarjito, Radjasa O.K, Hutabarat, S dan Prayitno S B., 2009. Phylogenetik Diversity of Causative Agent of Vibriosis Associated with Groupers Fish from Karimunjawa Island, Indonesia. Curr. Res. of Microbiol., 2 (1) : 14-21.

Sarjito, Radjasa O.K, Haditomo, Alfabetian. H. Condro, Prayitno S B., 2014. Insidensi Bakteri Genus Vibrio pPada Lele Dumbo (Clarias gariepinus) dari Sentral Produksi Provinsi Jawa Tengah. Seminar Nasional Ke-III Hasil-hasil Penelitian Perikanan dan Kelautan Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro.

Sarjito, Haditomo, Alfabetian. H. Condro, dan Prayitno S B., 2015. Causative Agent Vibriosis pada Ikan Lele Dumbo (Clarias gariepinus) yang Dibudidayakan di Kolam Beralinitas Rendah. Seminar Nasional Ke-IV Hasil-hasil Penelitian Perikanan dan Kelautan Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro.

Sharma A. and Chaturvedi, A.N., 2007. Population dynamic of Vibrio sp. in the river Narmahada at Jabalpur. J. Enviroment. Biol., 28 : 747 - 751

Sharma, A., C.R., Bora,C.R., Chaurasia, R.K., and Sahu, V., 2009. Antibiotic Suspectibility and Genetic Analysisi of Vibrio species Isolated from Reverine Enviroent. Curr. Res. Bacteriol., 19: 1 - 13

Sukenda, L. Jamal,D. Wahyuningrum dan A. Hasan. 2008. Penggunaan Kitosan Untuk Pencegahan Infeksi Aeromonas hydrophila Pada Ikan Lele Dumbo Clarias sp. Jurnal Akuakultur Indonesia, 7(2) : 159-169.

Tatsuya N., Emi I., Nakao N., Nobuhiko N., and Masatoshi M. 2006. Comparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection interms of toxicity and antibiotic resistance. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.




DOI: https://doi.org/10.31289/biolink.v5i1.1689

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